What's your protocol? What cloning system are you using? Which plasmid/vector system? Where are you getting your primers? How will you choose your primers? Do you know how to design the construct and primers so that it will anneal successfully? Which restriction enzymes are you using? Are you using the shampoo/isopropyl alcohol method for DNA prep? How will you quantitate your DNA? Where will you get antibiotic for your plates? How will you achieve FRET for melamine detection? (I'm a little fuzzy on how you're planning to link GFP to melamine deaminase and get it to emit). What kind of promoter will you use for the chimeric protein construct? Are you expecting a conformational change in the melamine deaminase upon ligand binding? I assume you're also planning to build a fluorescence detector? How will you confirm the presence of the cloned protein in your bacteria? Western blot? If so, will you also be building your own SDS-PAGE setup and western blot apparatus? How will you be validating your results? Where will you get melamine to test with? Are you planning to make yogurt with the resultant bacteria? Will you add antibiotic to the yogurt to maintain the plasmid? Is the idea to ultimately mix food into the yogurt and look for fluorescence with the naked eye?