As i stated in my initial post, it's the process of trading the benchtop for the kitchentop that intrigues me. You didn't inquire how she was going to clone her target, you wanted a methodology from start to finish. All I am suggesting is let her concentrate on the cloning before worrying about getting FRET to work in her recombinant bugs, validating her results, and packaging a final product!
As to how and if one can clone in their kitchen, I am certainly curious! MAKE magazine has already published plans for a homemade cycler, so if she can build a reliable electrophoresis rig, and put it all together with clean enough DNA preps, why couldn't she heat shock her vector into competent bugs? I should go make a username...
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As to how and if one can clone in their kitchen, I am certainly curious! MAKE magazine has already published plans for a homemade cycler, so if she can build a reliable electrophoresis rig, and put it all together with clean enough DNA preps, why couldn't she heat shock her vector into competent bugs? I should go make a username...