I think you're right, though -- I passed the pic on to the DIYbio folks who actually have degrees in this stuff, and they agree with your assessment. *facepalm* You live, you learn!
This makes me want to put together the synbio/bioinformatics equivalent of a syntax checker -- i.e., some way to specify the input organism, the input plasmid, the nutrient medium and the protocol by way of validating a dry-run in silico. We have input validation in computer science -- no reason not to have it in biology too.
Anyway, thanks for the comment -- you've been tremendous help, and I hope you see this comment some day, since I have no way of contacting you. :( Fortunately, the folks I talked to pointed me at a source for plasmids with the proper origin, so, onward and upward!
Re: ColE1 origin
I think you're right, though -- I passed the pic on to the DIYbio folks who actually have degrees in this stuff, and they agree with your assessment. *facepalm* You live, you learn!
This makes me want to put together the synbio/bioinformatics equivalent of a syntax checker -- i.e., some way to specify the input organism, the input plasmid, the nutrient medium and the protocol by way of validating a dry-run in silico. We have input validation in computer science -- no reason not to have it in biology too.
Anyway, thanks for the comment -- you've been tremendous help, and I hope you see this comment some day, since I have no way of contacting you. :( Fortunately, the folks I talked to pointed me at a source for plasmids with the proper origin, so, onward and upward!